Analysis Note

ControlHuman skeletal muscle tissue lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pink1 in 10 µg of human testis tissue lysate.

Immunohistochemistry Analysis: A representative lot detected Pink1 in rat caudate putamen tissue (Prof. J. Trimmer, University of California, Davis.).

Anti-Pink1 Antibody, clone N4/15 is a highly specific mouse monoclonal antibody & that targets PINK1 & has been tested in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mutations in the PTEN-induced kinase 1 (PINK1) gene located within the PARK6 locus on chromosome 1p35-p36 have recently been identified in patients with recessive early-onset Parkinson′s disease. Data suggests that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance.

Immunogen

Recombinant protein corresponding to the cytoplasmic domain of human Pink1.

Epitope: Cytoplasmic domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pink1 in 10 µg of human skeletal muscle tissue lysate.

Specificity

This antibody recognizes the cytoplasmic domain of Pink1. Other Homologies: Mouse (81% Sequence Homology) and Rat (82% Sequence Homology)

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Uniprot describes two isoforms at 63 kDa and 30 kDa The full length protein of Pink1 is observed at 63 kDa is the full length PINK1; however, it is proteolytically cleaved to a ~52 kDa form upon entry into the mitochondria (Jin, S. M., et al. (2010). J Cell Biol. 191(5):933-942.).